Application
CAI Yanmin, FAN Jiahuan, ZHANG Wei, LIN Wanling, LIU Hanxu, ZENG Zijian, SHI Jiale, CHEN Chudong, GUO Jiyun
To address the issues of requiring two separate instruments for traditional PCR and hybridization methods, as well as the problem of aerosol contamination, this paper presents an integrated nucleic acid PCR amplification and hybridization reactor. The device integrates PCR amplification and hybridization reactions into a single fully-enclosed system, mainly consisting of PCR amplification and hybridization reaction chip module, temperature control module, temperature acquisition and control system, main control board, and peristaltic pump module. Experimental results show that the instrument features simple structure, high temperature control precision, and accurate liquid transfer. It achieves automated control while effectively preventing aerosol contamination, demonstrating promising application prospects.